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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using XDH Rabbit mAb [PSW7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :XDH,XDHA,XDH_HUMAN,Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase ,Xanthine oxidase ]
UniProtID :P47989
MASS(da) :146,424
MW(kDa) :150KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit monoclonal IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human XDH.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro)..
Tissue specificity :Detected in milk (at protein level)..
Subcellular locationi :Cytoplasm. Peroxisome. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.