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Western blot(SDS PAGE) analysis of extracts from U2OS cells.Using OXGR1 Rabbit mAb [K1M9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :OXGR1,GPR80,GPR99,P2RY15,P2Y15,OXGR1_HUMAN,2-oxoglutarate receptor 1
UniProtID :Q96P68
MASS(da) :38,251
MW(kDa) : 40KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit monoclonal IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human OXGR1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U2OS cells
Function : Receptor for alpha-ketoglutarate. Seems to act exclusively through a G(q)-mediated pathway (By similarity)..
Tissue specificity :Detected in kidney and, to a lower extent, in placenta. Not detected in brain tissues including the frontal cortex, caudate putamen, thalamus, hypothalamus, hippocampus or pons.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.