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Western blot(SDS PAGE) analysis of extracts from DU145 cells.Using NPY5R Rabbit mAb [R144] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NPY5R,NPYR5,NPY5R_HUMAN,Neuropeptide Y receptor type 5
UniProtID :Q15761
MASS(da) :50,727
MW(kDa) : 50KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit IgG
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human NPY5R.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : DU145 cells.
Function : Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.
Tissue specificity :Brain,hypothalamus.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.