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Western blot analysis of extracts from Rat hippocampal homogenate tissue lyaste.using PGP9.5 Mouse mAb [64P1] at dilution of 1:1000 incubated at 4℃ over night
Protein names :UCHL1,UCHL1_HUMAN,Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProtID :P09936
MASS(da) :24,824
MW(kDa) :43 kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human PGP9.5.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Rat hippocampal homogenate tissue lyaste
Function : Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (PubMed:9774100, PubMed:8639624, PubMed:12408865, PubMed:23359680). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (PubMed:12408865)..
Tissue specificity :Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients..
Subcellular locationi :Cytoplasm. Endoplasmic reticulum membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.