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Western blot analysis of extracts from MCF-7 cells lysates grown in 20% FBS .using RPS6 (S240 + S244) Rabbit mAb [V4N7] at dilution of 1:1000 incubated at 4℃ over night
Protein names :RPS6,OK/SW-cl.2,RS6_HUMAN,40S ribosomal protein S6
UniProtID :P62753
MASS(da) :28,681
MW(kDa) :32 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser240 and Ser244 of Human RPS6 .
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells lysates grown in 20% FBS
Function : Component of the 40S small ribosomal subunit (PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279)..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.