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Western blot analysis of extracts from HeLa cells lysates treated with BMP-4 .using SMAD1+SMAD5+SMAD9 (S465+S467) Rabbit mAb [23PA] at dilution of 1:1000 incubated at 4℃ over night
Protein names :SMAD9,MADH6,MADH9,SMAD8,SMAD9_HUMAN,Mothers against decapentaplegic homolog 9
UniProtID :Q15797,Q99717,O15198
MASS(da) :52,493
MW(kDa) :52 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser465 and Ser467 of Human SMAD1+SMAD5+SMAD9 .
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells lysates treated with BMP-4
Function : Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Tissue specificity :Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.