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Western blot(SDS PAGE) analysis of extracts from C2C12 cells.Using HMGCS1 Rabbit mAb [J677] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HMGCS1,HMGCS,HMCS1_HUMAN,Hydroxymethylglutaryl-CoA synthase, cytoplasmic
UniProtID :Q01581
MASS(da) :57,294
MW(kDa) :57 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human HMGCS1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :C2C12 cells
Function : Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate, a precursor for cholesterol synthesis..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.