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Western blot analysis of extracts from HeLa cells lysates nocodazole-treated .using Histone H3.3 (S31) Rabbit mAb [RY41] at dilution of 1:1000 incubated at 4℃ over night
Protein names :H3-3A,H3.3A,H3F3,H3F3A,PP781,H3-3B,H3.3B,H3F3B,H33_HUMAN,Histone H3.3
UniProtID :P84243
MASS(da) :15,328
MW(kDa) :15 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser31 of Human Histone H3.3 .
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells lysates nocodazole-treated
Function : Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling..
Subcellular locationi :Nucleus. Chromosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.