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Western blot analysis of extracts from HeLa cells lyastes.using eEF1A1 + eEF1AL3 Rabbit mAb [ITZ4] at dilution of 1:1000 incubated at 4℃ over night
Protein names :Eef1a1,Eef1a,EF1A1_MOUSE,Elongation factor 1-alpha 1
UniProtID :P10126
MASS(da) :50,114
MW(kDa) :50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human eEF1A1 + eEF1AL3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells lyastes
Function : This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1..
Subcellular locationi :Cytoplasm. Nucleus. Nucleus, nucleolus. Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.