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PRKAR1A+PRKAR1B Rabbit mAb [7P99]Cat NO.: A52265

Western blot analysis of extracts from U-87 MG cells lyastes.using PRKAR1A+PRKAR1B Rabbit mAb [7P99] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :PRKAR1B,KAP1_HUMAN,cAMP-dependent protein kinase type I-beta regulatory subunit

UniProtID :P10644,P31321

MASS(da) :43,073

MW(kDa) :43 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human PRKAR1A+PRKAR1B.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :U-87 MG cells lyastes

Function : Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells..

Tissue specificity :Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Subcellular locationi :Cell membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.