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Western blot analysis of extracts from Fetal heart tissue lyaste.using ST3GAL3 Rabbit mAb [6G8Q] at dilution of 1:1000 incubated at 4℃ over night
Protein names :ST3GAL3,SIAT6,SIAT6_HUMAN,CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase GlcNAc alpha-2,3 sialyltransferase)
UniProtID :Q11203
MASS(da) :42,171
MW(kDa) :42 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human ST3GAL3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Fetal heart tissue lyaste
Function : Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc..
Tissue specificity :Highly expressed in adult skeletal muscle and in all fetal tissues examined and to a much lesser extent in placenta, lung and liver.
Subcellular locationi :Golgi apparatus, Golgi stack membrane,Single-pass type II membrane protein. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.