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FNDC5 Rabbit mAb [F0J4]Cat NO.: A77713

Western blot analysis of extracts from Mouse Skeletal Muscle tissue lyaste.using FNDC5 Rabbit mAb [F0J4] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :FNDC5,FRCP2,FNDC5_HUMAN,Fibronectin type III domain-containing protein 5 [Cleaved into: Irisin]

UniProtID :Q8NAU1

MASS(da) :23,659

MW(kDa) :24 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human FNDC5.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Mouse Skeletal Muscle tissue lyaste

Function : [Irisin]: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue..

Tissue specificity :Widely expressed, with highest levels in heart. Very low expression, if any, in colon, pancreas and spleen..

Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Peroxisome membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.