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Western blot analysis of extracts from HeLa cells lyastes.using NDUFS8 Rabbit mAb [7S5Y] at dilution of 1:1000 incubated at 4℃ over night
Protein names :NDUFS8,NDUS8_HUMAN,NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
UniProtID :O00217
MASS(da) :23,705
MW(kDa) :23 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human NDUFS8.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells lyastes
Function : Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:22499348). Essential for the catalytic activity and assembly of complex I (PubMed:22499348)..
Tissue specificity :Expressed in all tissues with the highest level in heart and skeletal muscle and the lowest level in lung..
Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein,Matrix side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.