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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using ApoA4 mouse mAb[L423] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :APOA4,APOA4_HUMAN,Apolipoprotein A-IV
UniProtID :P06727
MASS(da) :45,372
MW(kDa) :45 kDa
Form :Liquid
Purification :Protein A purification
Host :mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human ApoA4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II,potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Tissue specificity :Synthesized primarily in the intestine and secreted in plasma.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.