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Western blot analysis of extracts from Saos-2 cells lyastes.using CRTAP/CASP Rabbit mAb [10Q5] at dilution of 1:1000 incubated at 4℃ over night
Protein names :CRTAP,CASP,CRTAP_HUMAN,Cartilage-associated protein
UniProtID :O75718
MASS(da) :46,562
MW(kDa) :47 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CRTAP/CASP.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Saos-2 cells lyastes
Function : Necessary for efficient 3-hydroxylation of fibrillar collagen prolyl residues..
Tissue specificity :Found in articular chondrocytes. Expressed in a variety of tissues.
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.