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Western blot analysis of extracts from SH-SY5Y cells lyastes.using TPK1 Rabbit mAb [2PIH] at dilution of 1:1000 incubated at 4℃ over night
Protein names :TPK1,TPK1_HUMAN,Thiamin pyrophosphokinase 1
UniProtID :Q9H3S4
MASS(da) :27,265
MW(kDa) :27 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human TPK1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cells lyastes
Function : Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate..
Tissue specificity :Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.