支持:13376277882 | tech@naturebios.com

CAND1 Rabbit mAb [SGY7]Cat NO.: A10550

Western blot analysis of extracts from Jurkat cells lyastes.using CAND1 Rabbit mAb [SGY7] at dilution of 1:1000 incubated at 4℃ over night

  • SizePriceIn stock
  • 50ul1280咨询客服
  • 100ul2380咨询客服
订单 Datasheet

Product information

Protein names :CAND1,KIAA0829,TIP120,TIP120A,CAND1_HUMAN,Cullin-associated NEDD8-dissociated protein 1

UniProtID :Q86VP6

MASS(da) :136,376

MW(kDa) :136 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CAND1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Jurkat cells lyastes

Function : Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes..

Subcellular locationi :Cytoplasm. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.