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Western blot analysis of extracts from HACAT cells lyastes.using Periplakin Rabbit mAb [0W2E] at dilution of 1:1000 incubated at 4℃ over night
Protein names :PPL,KIAA0568,PEPL_HUMAN,Periplakin
UniProtID :O60437
MASS(da) :204,747
MW(kDa) :195 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Periplakin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HACAT cells lyastes
Function : Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling..
Tissue specificity :Expressed in stratified squamous epithelia and in some other epithelia..
Subcellular locationi :Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.