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Western blot analysis of extracts from HL60 cells lyastes.using Ube1L Rabbit mAb [DG4A] at dilution of 1:1000 incubated at 4℃ over night
Protein names :UBA7,UBE1L,UBE2,UBA7_HUMAN,Ubiquitin-like modifier-activating enzyme 7
UniProtID :P41226
MASS(da) :111,694
MW(kDa) :111 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Ube1L.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HL60 cells lyastes
Function : Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein..
Tissue specificity :Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.