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Western blot analysis of extracts from MCF-7 cells lyastes.using p50 dynamitin Rabbit mAb [YA50] at dilution of 1:1000 incubated at 4℃ over night
Protein names :DCTN2,DCTN50,DCTN2_HUMAN,Dynactin subunit 2
UniProtID :Q13561
MASS(da) :44,231
MW(kDa) :44 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human p50 dynamitin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells lyastes
Function : Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
Subcellular locationi :Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.