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Western blot analysis of extracts from Raji cells lyastes.using CD53 Rabbit mAb [2A55] at dilution of 1:1000 incubated at 4℃ over night
Protein names :CD53,MOX44,TSPAN25,CD53_HUMAN,Leukocyte surface antigen CD53
UniProtID :P19397
MASS(da) :24,341
MW(kDa) :24 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human CD53.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raji cells lyastes
Function : Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity)..
Tissue specificity :B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
Subcellular locationi :Cell membrane. Cell junction. Membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.