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Western blot analysis of extracts from 293T cells lyastes.using Ube4a Rabbit mAb [Y1OD] at dilution of 1:1000 incubated at 4℃ over night
Protein names :UBE4A,KIAA0126,UBE4A_HUMAN,Ubiquitin conjugation factor E4 A
UniProtID :Q14139
MASS(da) :122,561
MW(kDa) :123 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Ube4a.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells lyastes
Function : Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.