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BIG-2 Rabbit mAb [EQ85]Cat NO.: A44327

Western blot analysis of extracts from Human fetal brain tissue lyaste.using BIG-2 Rabbit mAb [EQ85] at dilution of 1:1000 incubated at 4℃ over night

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Product information

Protein names :CNTN4,CNTN4_HUMAN,Contactin-4

UniProtID :Q8IWV2

MASS(da) :113,454

MW(kDa) :113 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human BIG-2.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Human fetal brain tissue lyaste

Function : Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.

Tissue specificity :Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex..

Subcellular locationi :Cell membrane,Lipid-anchor, GPI-anchor. Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.