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Western blot analysis of extracts from Human fetal brain tissue lyaste.using BIG-2 Rabbit mAb [EQ85] at dilution of 1:1000 incubated at 4℃ over night
Protein names :CNTN4,CNTN4_HUMAN,Contactin-4
UniProtID :Q8IWV2
MASS(da) :113,454
MW(kDa) :113 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human BIG-2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal brain tissue lyaste
Function : Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.
Tissue specificity :Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex..
Subcellular locationi :Cell membrane,Lipid-anchor, GPI-anchor. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.