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Western blot analysis of extracts from Fetal heart tissue lyaste.using TNNC1 Rabbit mAb [4P4O] at dilution of 1:1000 incubated at 4℃ over night
Protein names :TNNC1,TNNC,TNNC1_HUMAN,Troponin C, slow skeletal and cardiac muscles
UniProtID :P63316
MASS(da) :18,403
MW(kDa) :18 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human TNNC1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Fetal heart tissue lyaste
Function : Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.