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Western blot analysis of extracts from C6 cells lyastes.using TyrRS Rabbit mAb [N849] at dilution of 1:1000 incubated at 4℃ over night
Protein names :YARS1,YARS,SYYC_HUMAN,Tyrosine--tRNA ligase, cytoplasmic [Cleaved into: Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed]
UniProtID :P54577
MASS(da) :59,143
MW(kDa) :59 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human TyrRS.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :C6 cells lyastes
Function : Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.