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Western blot analysis of extracts from PC-3 cells lyastes.using uPA Rabbit mAb [2B72] at dilution of 1:1000 incubated at 4℃ over night
Protein names :PLAU,UROK_HUMAN,Urokinase-type plasminogen activator [Cleaved into: Urokinase-type plasminogen activator long chain A,Urokinase-type plasminogen activator short chain A,Urokinase-type plasminogen activator chain B]
UniProtID :P00749
MASS(da) :48,523
MW(kDa) :48 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human uPA.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :PC-3 cells lyastes
Function : Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
Tissue specificity :Expressed in the prostate gland and prostate cancers..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.