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Western blot analysis of extracts from Fetal brain tissue lyaste.using ME3 Rabbit mAb [4V0T] at dilution of 1:1000 incubated at 4℃ over night
Protein names :ME3,MAON_HUMAN,NADP-dependent malic enzyme, mitochondrial
UniProtID :Q16798
MASS(da) :67,068
MW(kDa) :67 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human ME3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Fetal brain tissue lyaste
Function : Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+) as a cofactor (PubMed:7818469). Can also reverse the decarboxylation reaction, but only with significantly lower efficiency (PubMed:7818469)..
Tissue specificity :Expressed predominantly in organs with a low-division rate.
Subcellular locationi :Mitochondrion matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.