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Western blot analysis of extracts from Human small intestine tissue lyaste.using αDefensin 5 Rabbit mAb [BV46] at dilution of 1:1000 incubated at 4℃ over night
Protein names :DEFA5,DEF5,DEF5_HUMAN,Defensin-5 ) [Cleaved into: HD5,HD5,HD5,HD5]
UniProtID :Q01523
MASS(da) :10,071
MW(kDa) :10 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human αDefensin 5.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human small intestine tissue lyaste
Function : Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing..
Tissue specificity :Paneth cells of the small intestine (at protein level)..
Subcellular locationi :Secreted. Cytoplasmic vesicle, secretory vesicle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.