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Western blot analysis of extracts from Neuro-2a cells lyastes.using AER61 Rabbit mAb [X5IT] at dilution of 1:1000 incubated at 4℃ over night
Protein names :EOGT,AER61,C3orf64,EOGT1,EOGT_HUMAN,EGF domain-specific O-linked N-acetylglucosamine transferase
UniProtID :Q5NDL2
MASS(da) :62,011
MW(kDa) :62 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human AER61.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Neuro-2a cells lyastes
Function : Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains..
Subcellular locationi :Endoplasmic reticulum lumen.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.