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Western blot analysis of extracts from T47D cells lyastes.using NDUFAF4 Rabbit mAb [9Z4B] at dilution of 1:1000 incubated at 4℃ over night
Protein names :NDUFAF4,C6orf66,HRPAP20,HSPC125,My013,NDUF4_HUMAN,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
UniProtID :Q9P032
MASS(da) :20,266
MW(kDa) :20 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human NDUFAF4.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :T47D cells lyastes
Function : Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I) (PubMed:18179882, PubMed:28853723). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion..
Subcellular locationi :Mitochondrion. Membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.