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Western blot analysis of extracts from Mouse eyeball tissue lyaste.using LIM2 Rabbit mAb [MZL4] at dilution of 1:1000 incubated at 4℃ over night
Protein names :LIM2,LMIP_HUMAN,Lens fiber membrane intrinsic protein
UniProtID :P55344
MASS(da) :19,674
MW(kDa) :20 kda
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human LIM2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse eyeball tissue lyaste
Function : Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis.
Tissue specificity :Eye lens specific..
Subcellular locationi :Membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.