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Western blot analysis of extracts from Human pancreas tissue lyaste.using SPINK1/P12 Rabbit mAb [VCBO] at dilution of 1:1000 incubated at 4℃ over night
Protein names :SPINK1,PSTI,ISK1_HUMAN,Serine protease inhibitor Kazal-type 1
UniProtID :P00995
MASS(da) :8,507
MW(kDa) :11 kda
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human SPINK1/P12.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human pancreas tissue lyaste
Function : Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (By similarity).., In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.