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Western blot analysis of extracts from HepG2 cells lyastes.using ALDH7A1 Rabbit mAb [6T3R] at dilution of 1:1000 incubated at 4℃ over night
Protein names :ALDH7A1,ATQ1,AL7A1_HUMAN,Alpha-aminoadipic semialdehyde dehydrogenase
UniProtID :P49419
MASS(da) :58,487
MW(kDa) :55 kda
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human ALDH7A1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells lyastes
Function : Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism..
Tissue specificity :Abundant in hepatoma cells and fetal cochlea, ovary, eye, heart, adrenal gland, liver and kidney. Low levels present in adult peripheral blood leukocytes and fetal brain, thymus, spleen, skeletal muscle, lung and tongue..
Subcellular locationi :[Isoform 2]: Cytoplasm, cytosol. Nucleus.,[Isoform 1]: Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.