HMGCR Rabbit mAb [7ELZ]Cat NO.: A34218

Protein names ：HMGCR,HMDH_HUMAN,3-hydroxy-3-methylglutaryl-coenzyme A reductase

UniProtID ：P04035

MASS(da) ：97,476

MW(kDa) ：100kDa

Form ：Rabbit IgG

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of Human HMGCR.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：293T cells lysate

Function ： Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis (PubMed:2991281, PubMed:21357570, PubMed:6995544). HMGCR is the main target of statins, a class of cholesterol-lowering drugs (PubMed:11349148, PubMed:18540668)..

Tissue specificity ：[Isoform 1]: Ubiquitously expressed with the highest levels in the cerebellum, fetal brain, testis, skin and adrenal gland..,[Isoform 2]: Detected in the cerebellum, fetal brain, testis and adrenal gland..,[Isoform 3]: Low abundance except in skin, esophagus, and uterine cervix..

Subcellular locationi ：Endoplasmic reticulum membrane,Multi-pass membrane protein. Peroxisome membrane,Multi-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.