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Western blot(SDS-PAGE) analysis of extracts from Mouse skin tissue lysate.using Mast Cell Tryptase Rabbit mAb [36WM] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TPSB2,TPS2,TRYB2_HUMAN,Tryptase beta-2
UniProtID :P20231
MASS(da) :30,515
MW(kDa) :31KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Mast Cell Tryptase.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse skin tissue lysate
Function : Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.