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Western blot(SDS-PAGE) analysis of extracts from HeLa cells lysate.using Nesprin3 Rabbit mAb [V7BZ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SYNE3,C14orf139,C14orf49,LINC00341,SYNE3_HUMAN,Nesprin-3
UniProtID :Q6ZMZ3
MASS(da) :112,216
MW(kDa) :112KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity : Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Human Nesprin3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : HeLa cells lysate
Function : As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells..
Tissue specificity :Expressed in aortic endothelial cells (at protein level)..
Subcellular locationi :Nucleus outer membrane,Single-pass type IV membrane protein. Nucleus envelope. Rough endoplasmic reticulum.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.