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Western blot(SDS-PAGE) analysis of extracts from NIH/3T3 cells lysate Paclitaxel-treated.using Cdc6 (S54) Rabbit mAb [V1CL] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDC6,CDC18L,CDC6_HUMAN,Cell division control protein 6 homolog )
UniProtID :Q99741
MASS(da) :62,720
MW(kDa) : 63kDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity : Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around S54 of Human Cdc6.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells lysate Paclitaxel-treated
Function : Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.