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CRK/CRKII (Y221) Rabbit mAb [44N4]Cat NO.: A10381

Western blot(SDS-PAGE) analysis of extracts from NIH/3T3 cells lysate CA-treated.using CRK/CRKII (Y221) Rabbit mAb [44N4] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CRK,CRK_HUMAN,Adapter molecule crk

UniProtID :P46108

MASS(da) :33,831

MW(kDa) : 34KDa

Form :Rabbit IgG

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity : Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Y221 of Human CRK/CRKII.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :NIH/3T3 cells lysate CA-treated

Function : Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.., [Isoform Crk-II]: Regulates cell adhesion, spreading and migration (PubMed:31311869). Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4 (PubMed:19004829). May regulate the EFNA5-EPHA3 signaling (By similarity)..

Subcellular locationi :Cytoplasm. Cell membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.