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Western blot(SDS-PAGE) analysis of extracts from NIH/3T3 cells lysate CA-treated.using CRK/CRKII (Y221) Rabbit mAb [44N4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CRK,CRK_HUMAN,Adapter molecule crk
UniProtID :P46108
MASS(da) :33,831
MW(kDa) : 34KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity : Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Y221 of Human CRK/CRKII.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells lysate CA-treated
Function : Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.., [Isoform Crk-II]: Regulates cell adhesion, spreading and migration (PubMed:31311869). Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4 (PubMed:19004829). May regulate the EFNA5-EPHA3 signaling (By similarity)..
Subcellular locationi :Cytoplasm. Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.