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Western blot(SDS-PAGE) analysis of extracts from NIH/3T3 cells lysate Nocodazole-treated.using Histone H1.3 (T17)/Histone H1.4 (T17) Rabbit mAb [LOA1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :H1-3,H1F3,HIST1H1D,H13_HUMAN,Histone H1.3
UniProtID :P16402
MASS(da) :22,350
MW(kDa) :30KDa
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around T17 of Human Histone H1.3)/Histone H1.4.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells lysate Nocodazole-treated
Function : Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity)..
Subcellular locationi :Nucleus. Chromosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.