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Western blot(SDS-PAGE) analysis of extracts from NIH/3T3 cells lysate Anisomycin-treated.using JunD (S255) Rabbit mAb [62J6] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :JUND,JUND_HUMAN,Transcription factor JunD
UniProtID :P17535
MASS(da) :35,174
MW(kDa) : 38KDa/42Kda
Form :Rabbit IgG
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity : Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around S255 of Human JunD.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells lysate Anisomycin-treated
Function : Transcription factor binding AP-1 sites (PubMed:9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed:9989505, PubMed:28981703)..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.