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Western blot(SDS PAGE) analysis of extracts from HeLa acid extract Cells lysate.Using Tri-Methyl-Histone H3 (Lys4) Rabbit mAb [IW01] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :H3C1,H3FA,HIST1H3A,H3C2,H3FL,HIST1H3B,H3C3,H3FC,HIST1H3C,H3C4,H3FB,HIST1H3D,H3C6,H3FD,HIST1H3E,H3C7,H3FI,HIST1H3F,H3C8,H3FH,HIST1H3G,H3C10,H3FK,HIST1H3H,H3C11,H3FF,HIST1H3I,H3C12,H3FJ,HIST1H3J,H31_HUMAN,Histone H3.1
UniProtID :Q16695
MASS(da) :15,404
MW(kDa) :17kda
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat,Mouse
Specificity :Antibody is produced by immunizing animals with A synthetic trimethylated peptide around K4 of human Histone H3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa acid extract Cells
Function : Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular locationi :Nucleus. Chromosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.