Notch3 Rabbit mAb [5J0A]Cat NO.: A25639

Protein names ：NOTCH3,NOTC3_HUMAN,Neurogenic locus notch homolog protein 3 [Cleaved into: Notch 3 extracellular truncation,Notch 3 intracellular domain]

UniProtID ：Q9UM47

MASS(da) ：243,631

MW(kDa) ：90, 270 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Notch3

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：MCF-7 cells

Function ： Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination (PubMed:15350543). Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity)..

Tissue specificity ：Ubiquitously expressed in fetal and adult tissues.

Subcellular locationi ：Cell membrane,Single-pass type I membrane protein.,[Notch 3 intracellular domain]: Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.