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Western blot(SDS PAGE) analysis of extracts from RAW264.7 cell lysate.Using KMT4 / Dot1L Rabbit mAb [X26G]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DOT 1; DOT1L; KMT4; Histone methyltransferase DOT1L; H3 lysine-79 specific;
UniProtID :Q8TEK3
MASS(da) :164,856
MW(kDa) :185kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Mouse
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human KMT4 / Dot1L
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :RAW264.7 cell lysate.
Function : Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones (PubMed:12123582). Binds to DNA (PubMed:12628190)..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.