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MMP12 Rabbit mAb [wM5e]Cat NO.: A83820

Western blot(SDS PAGE) analysis of extracts from WI-38 cell lysate.Using MMP12 Rabbit mAb [wM5e]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :Macrophage metalloelastase; MME; 3.4.24.65; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12; MMP12; HME;

UniProtID :P39900

MASS(da) :54,002

MW(kDa) :54,45,22kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human MMP12

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :WI-38 cell lysate.

Function : May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

Tissue specificity :Found in alveolar macrophages but not in peripheral blood monocytes.

Subcellular locationi :Secreted, extracellular space, extracellular matrix.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.