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Western blot(SDS PAGE) analysis of extracts from (1) Human brain lysate; (2) Human brain lysate treated with AP.Using P-Synapsin I (S9) Rabbit mAb [J76y]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Brain protein 4.1; SYN-1; synapsin I;
UniProtID :P17600
MASS(da) :74,111
MW(kDa) :77kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Phospho-Synapsin I (S9)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) Human brain lysate; (2) Human brain lysate treated with AP.
Function : Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
Subcellular locationi :Cell junction, synapse. Golgi apparatus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.