CD147 Rabbit mAb [Qib2]Cat NO.: A42283

Protein names ：5A11 antigen; Basigin; Blood brain barrier HT7 antigen; Bsg; CD147; Collagenase stimulatory factor; EMMPRIN; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; Neurothelin;OK blood group; OK blood group antigen; TCSF; Tumor cell derived collagenase stimulatory factor;

UniProtID ：P18572(mouse)

MASS(da) ：42,445

MW(kDa) ：45-55kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Mouse

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human CD147

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：RAW 264.7 cell lysate.

Function ： [Isoform 1]: Essential for normal retinal maturation and development (PubMed:10967074, PubMed:11273674, PubMed:11853760). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687).., [Isoform 2]: Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (By similarity). Plays an important role in targeting the monocarboxylate transporters SLC16A1/GLUT1, SLC16A3 and SLC16A8 to the plasma membrane (PubMed:12601063). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (By similarity). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA and KDR/VEGFR2 in endothelial cells (By similarity). Plays an important role in spermatogenesis,mediates interactions between germ cells and Sertoli cell and is essential for the development/differentiation of germ cells to round spermatids (PubMed:23727514, PubMed:11882021)..

Tissue specificity ：[Isoform 1]: Retina-specific (PubMed:12939332, PubMed:11273674, PubMed:11853760, PubMed:25957687). Expressed in both rods and cones (at protein level) (PubMed:25957687)..,[Isoform 2]: Testis and caput, corpus and cauda epididymides (at protein level) (PubMed:11882021, PubMed:23727514, PubMed:21792931). Expressed in the brain, lung, liver, kidney, heart, spleen, uterus, retina and skeletal muscle (PubMed:2361961, PubMed:12939332, PubMed:12601063)..

Subcellular locationi ：[Isoform 1]: Cell membrane,Single-pass type I membrane protein. Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment.,[Isoform 2]: Cell membrane,Single-pass type I membrane protein. Endoplasmic reticulum membrane,Single-pass type I membrane protein. Basolateral cell membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.